Cloning and Characterization of a Novel Mannose-binding Protein of Acanthamoeba
نویسندگان
چکیده
منابع مشابه
Biochemical characterization and functional studies of Acanthamoeba mannose-binding protein.
Acanthamoebae produce a painful, sight-threatening corneal infection. The adhesion of parasites to the host cells is a critical first step in the pathogenesis of infection. Subsequent to adhesion, the parasites produce a potent cytopathic effect (CPE) leading to target cell death. Recent studies showing that acanthamoebae express a mannose-binding protein (MBP) and that free alpha-mannose (alph...
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background: acanthamoeba castellanii is the important cause of amoebic keratitis in iran. the key molecule in pathogenesis of acanthamoeba keratitis is mannose binding protein (mbp) led to adhesion of amoeba to corneal epithelium. subsequent to adhesion other cytopathic effects occur. the goal of this study was to identify the molecular characterization of a gene encoding mbp in an iranian is...
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background: acanthamoeba keratitis develops by pathogenic acanthamoeba such as a. palestinensis . indeed this species is one of the known causative agents of amoebic keratitis in iran. mannose binding protein (mbp) is the main pathogenicity factors for developing this sight threatening disease. we aimed to characterize mbp gene in pathogenic acanthamoeba isolates such as a. palestinensis . m...
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Using RNA extracted from Zantedeschia aethiopica young leaves and primers designed according to the conservative regions of Araceae lectins, the full-length cDNA of Z. aethiopica agglutinin (ZAA) was cloned by rapid amplification of cDNA ends (RACE). The full-length cDNA of zaa was 871 bp and contained a 417 bp open reading frame (ORF) encoding a lectin precursor of 138 amino acids. Through com...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2004
ISSN: 0021-9258
DOI: 10.1074/jbc.m402334200